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Combinatorial Chemistry & High Throughput Screening

Volume 11 Issue 7
ISSN: 1386-2073
eISSN: 1875-5402

 

   All Titles

  Fluorescent Probes for Cellular Assays
  pp.505-513 (9) Authors: George T. Hanson, Bonnie J. Hanson
 
 
      Abstract

A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. Fluorescent probes have been used for nearly a century to study cellular processes due to their exquisite sensitivity and selectivity. Fluorescent probes have also gained in popularity as safety and environmental concerns over the use of radioactive probes have grown. At the same time, cellular assays are being more widely used now than ever before. This review will give a broad overview of types of fluorescent probes, types of fluorescent assays, and their application in cellular assays for a number of pharmaceutically relevant target classes.

 
  Keywords: Fluorescence, cell based assays, high throughput screening, GPCR, pathway, ion channel, kinase, multiplexing.
  Affiliation: Invitrogen Discovery Sciences, 501 Charmany Drive, Madison, WI 53719, USA.
 
  Key: New Content Free Content Open Access Plus Subscribed Content

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