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Current Proteomics

Volume 8 Issue 3
ISSN: 1570-1646
eISSN: 1875-6247

 

   All Titles

  Abundance- and Activity-Based Proteomics in Platelet Biology
  pp.216-228 (13) Authors: Stephen P. Holly, Xian Chen, Leslie V. Parise
 
 
      Abstract

Human platelets are thought to express approximately 2000-3000 proteins, but post-translational modifications, alternatively spliced variants and a rich diversity of vertebrate domain architectures likely make this a conservative estimate. Even though rapidly advancing proteomic techniques have catalyzed the identification of roughly one third of the platelet proteome, a combination of abundance-based and activity-based proteomics is needed for elucidation of platelet functional characteristics including the definition of a “core proteome” and recognition of diverse enzyme activity profiles associated with various physiological states. In this review, we describe the latest mass spectrometry-based techniques capable of providing some of these physiological details required for more comprehensive evaluation of the human platelet repertoire.

 
  Keywords: ABPP, biomarker, cancer, library, mass spectrometry, platelet, proteome, rat, hemostasis, interaction, interactome, protein, thrombosis, megakaryocyte, low-abundant proteins, cytoskeleton, vasodilator-stimulated, phosphorylations, kinase substrates, Glanzmann thrombasthenia
  Affiliation: Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7260, USA.
 
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