Guest Open Access Plus | Free Content | About | Sign in | New Users: Sign up | Mark List  

Current Stem Cell Research & Therapy

Volume 5 Issue 3
ISSN: 1574-888X
eISSN: 2212-3946

 

   All Titles

  Hepatic Endoderm Differentiation from Human Embryonic Stem Cells
  pp.233-244 (12) Authors: Zara Hannoun, Celine Filippi, Gareth Sullivan, David C. Hay, John P. Iredale
 
 
      Abstract

Primary human hepatocytes are a scarce resource with variable function which diminishes with time in culture. As a consequence their use in tissue modeling and therapy is restricted. Human embryonic stem cells (hESCs) could provide a stable source of human tissue due to their properties of self-renewal and their ability to give rise to all three germ layers. hESCs have the potential to provide an unlimited supply of hepatic endoderm (HE) which could offer efficient tools for drug discovery, disease modeling and therapeutic applications. There has been a major focus on developing protocols to derive functional HE from hESCs. This review focuses on human liver biology and the translation of observations of in vivo systems into developing differentiation protocols to yield hepatic endoderm. It also details the potential role of oxygen tension as a new regulatory mechanism in HE differentiation and points out the importance of mitochondrial function analysis in defining successful HE generation.

 
  Keywords: Human embryonic stem cells, liver, endoderm, mitochondria, hypoxia
  Affiliation: Centre for Inflammation Research, University of Edinburgh, QMRI, 47 Little France crescent, Edinburgh EH16 4SB, UK.
 
  Key: New Content Free Content Open Access Plus Subscribed Content

Bentham Science Publishers
www.benthamscience.com

 

  Copyright © 1994 - 2014   Bentham Science Publishers