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The Natural Products Journal

Volume 1 Issue 2
ISSN: 2210-3155
eISSN: 2210-3163

 

   All Titles

  HPLC Analysis of Polysaccharides in Quince (Cydonia Oblonga Mill. var. maliformis) Fruit and PTP1B Inhibitory Activity
  pp.146-150 (5) Authors: Hajinisha Hopur, Akmal M. Asrorov, Ma Qingling, Ablimit Yili, Amatjan Ayupbek, Pang Nannan, Haji A. Aisa
doi: 10.2174/10146
 
 
      Abstract

A high performance liquid chromatography (HPLC) method for the determination of the monosaccharide compositions and molar ratio of polysaccharide in quince has been established. The quince polysaccharides were derivatized with 1-phenyl-3-methyl-5- pyrazolone (PMP) after hydrolyzed into monosaccharides with 2 M sulfuric acid. Monosaccharide derivatives were separated by a reversed phase C18 column and monitored by UV at 250 nm. Analysis of monosaccharides of quince polysaccharides sample by HPLC indicated the presence of mannose, rhamnose, glucose, galactose, and arabinose with the molar ratio of 0.10, 0.72, 1.12, 4.23, and 4.73, respectively. It could be applied for the quality control of quince polysaccharides. Moreover, quince polysaccharide shows the inhibitory activity of protein tyrosine phosphatase- 1B (PTP1B) with IC50 value of 2.07 μg/mL.

 
  Keywords: Derivatization, high performance liquid chromatography, Analysis, 1-phenyl-3-methyl-5-pyrazolone, Polysaccharide of quince, protein tyrosine phosphatase-1B, Inhibitory activity, Hydrolysed, Monosaccharide composition, UV
  Affiliation: Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, People's Republic of China.
 
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