Guest Open Access Plus | Free Content | About | Sign in | New Users: Sign up | Mark List  

Protein & Peptide Letters

Volume 14 Issue 3
ISSN: 0929-8665
eISSN: 1875-5305

 

   All Titles

  Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner
  pp.265-268 (4) Authors: Jikui Song, John L. Markley, Jikui Song, John L. Markley
 
 
      Abstract

As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.

 
  Keywords: Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions
  Affiliation: Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA., , Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA.
 
  Key: New Content Free Content Open Access Plus Subscribed Content

Bentham Science Publishers
www.benthamscience.com

 

  Copyright © 1994 - 2014   Bentham Science Publishers