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Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner
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pp.265-268 (4) Authors: Jikui Song, John L. Markley, Jikui Song, John L. Markley
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| Abstract |
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As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.
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Keywords:
Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions
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Affiliation:
Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA., , Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA.
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