All
Titles
|
| |
Stability Check of Succinylated Concanavalin A: Presence of Functionally Active Conformational State
|
| |
pp.423-429 (7) Authors: Sadaf Fatima, Rizwan Hasan Khan
|
|
| |
|
| Abstract |
| |
The equilibrium denaturation pathway of Succinyl Con A exhibited three-state mechanism with the transition midpoints at 1 and 3 M GdnHCl and at 2.6 and 5 M urea. Unfolding resulted in stabilization of molten-globule (MG) like intermediate states at 2 M GdnHCl and 3 M urea. It was particularly interesting that state obtained at 3 M urea was functionally active. |
|
| |
Keywords:
Succinylated Con A, Molten Globule like state, equilibrium denaturation, guanidine hydrochloride, urea |
| |
Affiliation:
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh - 202002, India. |
| |
|
|
